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. Author manuscript; available in PMC: 2024 Sep 1.
Published in final edited form as: Biomaterials. 2023 Jun 14;300:122201. doi: 10.1016/j.biomaterials.2023.122201

Figure 2. Multiple Gelation Mechanisms of SilkVS.

Figure 2.

(A) SilkVS has the potential to crosslink via three distinct mechanisms: thiol-ene Michael-type addition (green) occurred in the presence of a dithiol molecule (DTT), enzymatically induced dityrosine formation (orange) in the presence of horseradish peroxidase (HRP) and H2O2, and photocrosslinking (blue) in the presence of a photoinitiator (LAP) and 400nm blue light. (B) The gelation kinetics for each mechanism were tracked via in situ rheometry to monitor shear modulus (n=3). (C) Compressive moduli of the hydrogels were measured (n=5). All data presented are means ± SDs.; * p≤0.05, ** p≤0.01, *** p≤0.005, **** p<0.0001 No significance unless otherwise indicated.