Figure 3. SilkVS Hydrogel Cytocompatibility.

(A) Cytocompatibility was assessed by seeding fibroblasts on enzymatically crosslinking SilkVS and native silk hydrogels and observing metabolic activity and viability. (B) Cell metabolism was assessed using AlamarBlue over 7 days (n=5). (C) Cell viability was measured with fluorescence imaging where live cells were indicated by a green signal and cell death by red signal (C). Images are representative (n=5) All data presented are means ± SDs.; * p≤0.05, ** p≤0.01, *** p≤0.005, **** p<0.0001. No significance unless otherwise indicated.