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. 2023 Jun 23;299(8):104950. doi: 10.1016/j.jbc.2023.104950

Figure 5.

Figure 5

Abnormal spindle formation occurs in defolliculated epidermal growth factor receptor (EGFR)–Grb7-expressing oocytes that display germinal vesicle breakdown (GVBD) acceleration.Xenopus defolliculated oocytes (DEs) and folliculated oocytes (FCs) expressing EGFR were injected or not with Grb7 (20 ng) and incubated or not with 500 nM of integrin inhibitor echistatin (Echist) for DE or with Grb7 (20 ng) with or without 10 μM of pyrintegrin activator (Int Act) for FC, 1 h before EGF stimulation (5 nM); controls were left unstimulated. Naïve oocytes were treated with progesterone (PG). Oocytes were fixed, dehydrated, and paraffin-embedded before sections (7 μm) were stained with nuclear red to detect nuclei and chromosomes and picroindigocarmine to reveal cytoplasmic structures. The normal, abnormal, or absent spindles were scored. Cortical pigment (CP), plasma membrane (PM), and vitelline platelet (VP); the arrow points to the chromosomes on the spindle, and the scale bar represents 35 μm. Statistical significance against DE or FC was accepted for ∗∗∗∗p < 0.001.