Figure 6.

Favoring O-GlcNAcylation by O-GlcNAcase (OGA) inhibition lowers GVBD₅₀in defolliculated oocytes (DEs). Xenopus DE and folliculated oocytes (FCs), expressing epidermal growth factor receptor (EGFR), were microinjected with Grb7 (15 ng) or not, incubated or not with 50 or 100 μM OGA inhibitor Thiamet G (ThG), 1 h before EGF stimulation (5 nM). DE oocytes were submitted to integrin inhibitor echistatin 500 nM (Echist). A and B, the germinal vesicle breakdowns (GVBDs) were scored. C and D, GVBD₅₀ (time of half-responsive FC oocytes to undergo GVBD) were determined. Results were obtained on 20 oocytes from three different females. Statistical significance was accepted for ∗∗p < 0.01 and ∗∗∗∗p < 0.001. GVBD_50, half-maximal time attesting for a maturation.