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. 2023 Jun 20;299(8):104945. doi: 10.1016/j.jbc.2023.104945

Figure 4.

Figure 4

Frankenbodies validate hFWE isoform structure on organellar and vesicular membranes. HEK293 cells stably expressing EGFP alone, or coexpressing non-fused EGFP and hFWE3 (A) or hFWE4 (B) harboring epitope tags at indicated positions were transduced with lentivirus encoding HA- or FLAG-targeting Frankenbodies. Transduced cells were live imaged for native EGFP (green) and mCherry/mRuby2 fluorescence (magenta). Representative max intensity projections of central Z-slices are presented from 100× live cell confocal images, scale bar = 10 μm. C, schematic overview of hFWE isoform structure on intracellular membranes, with hFWE4 assuming a four transmembrane pass structure and hFWE3 assuming a two transmembrane pass structure. Both isoforms position N and C termini cytosolically.