Figure 5.

Total expression and surface expression of KCNQ2 channel. (A) Western blot analysis of proteins from total lysates (top) or plasma membrane fractions (bottom) from the negative control (NC, non-transfected CHO cells) or the CHO cells transfected with KCNQ2 variants. (B) Protein expression levels in total lysates obtained from transfected CHO cells were determined by normalizing KCNQ2 variants to the corresponding actin signals in four independent experiments. (C) Surface expression levels of the KCNQ2 variants normalized to WTKCNQ2 from four independent experiments. Statistically significant differences *p < 0.05 versus WT KCNQ2.