Table 2.

Biophysical properties of mutant KCNQ2/3 channel.

	Current density (pA/pF)	V0.5 (mV)	K (mV/e-Fold)
Q2 WT	81.0 ± 6.7	−23.1 ± 0.5	11.8 ± 0.4
A185T	45.7 ± 10.2a	−11.8 ± 0.7a	11.6 ± 0.6
G215R	3.1 ± 1.2a	—	—
I238L	73.6 ± 8.0	−6.4 ± 1.0a	11.0 ± 0.8
A265V	2.0 ± 0.9a	—	—
T277A	5.5 ± 1.2a	—	—
P285S	4.0 ± 1.1a	—	—
G290S	3.4 ± 1.7a	—	—
G301S	2.3 ± 1.0a	—	—
D563N	6.4 ± 1.3a	—	—
M578I	7.2 ± 2.2a	—	—
K606X	2.4 ± 1.0a	—	—
Q2+A185T	52.0 ± 9.1a	−12.4 ± 1.0a	11.7 ± 0.6
Q2+G215R	29.4 ± 6.4a	−16.4 ± 1.2a	11.4 ± 0.5
Q2+I238L	73.9 ± 8.2	−15.5 ± 1.1a	10.3 ± 1.0
Q2+A265V	24.7 ± 6.9a	−11.2 ± 1.2a	12.5 ± 0.8
Q2+T277A	26.5 ± 8.2a	−17.3 ± 0.8a	10.1 ± 0.5
Q2+P285S	23.5 ± 3.6a	−19.0 ± 0.8a	8.2 ± 0.5a
Q2+G290S	11.3 ± 4.5a	−16.8 ± 1.4a	9.4 ± 0.6
Q2+G301S	20.6 ± 5.0a	−17.9 ± 0.9a	9.5 ± 0.6
Q2+D563N	15.1 ± 4.0a	−10.0 ± 1.4a	11.5 ± 0.6
Q2+M578I	20.8 ± 1.3a	−16.0 ± 1.0a	9.7 ± 1.0
Q2+K606X	51.1 ± 11.3a	−16.7 ± 0.9a	7.1 ± 0.6a
Q2 WT+Q3 WT (1:1)	281.6 ± 18.7	−24.9 ± 0.6	10.7 ± 0.4
Q2 WT+Q3 WT (0.5:1)	202.9 ± 35.3b	−26.3 ± 0.9	9.7 ± 0.6
Q2+A185T+Q3	200.9 ± 23.1b	−21.2 ± 1.0c	8.6 ± 0.7
Q2+G215R+Q3	139.6 ± 9.6b,c	−17.7 ± 0.9b,c	9.0 ± 1.0
Q2+I238L+Q3	270.4 ± 23.5c	−20.5 ± 0.8b,c	10.8 ± 0.6
Q2+A265V+Q3	145.2 ± 26.3b,c	−12.1 ± 1.2b,c	11.8 ± 0.9
Q2+T277A+Q3	195.3 ± 35.8b	−15.4 ± 0.8b,c	10.0 ± 1.0
Q2+P285S+Q3	205.2 ± 20.9b	−21.8 ± 1.0c	9.2 ± 0.8
Q2+G290S+Q3	71.6 ± 12.3b,c	−18.7 ± 0.6b,c	9.6 ± 0.6
Q2+G301S+Q3	191.8 ± 25.2b	−18.4 ± 0.7b,c	11.9 ± 0.5
Q2+D563N+Q3	150.5 ± 17.3b,c	−17.4 ± 1.0b,c	9.4 ± 1.0
Q2+M578I+Q3	268.2 ± 32.2c	−22.0 ± 0.8c	8.4 ± 0.8
Q2+K606X+Q3	294.3 ± 30.5c	−21.0 ± 0.7b,c	9.6 ± 0.9

Current densities were calculated as peak current at +40 mV. V_0.5 is the voltage of half-maximal activation; k is the slope factor. Data are presented as means ± SEM, n = 8–15.

^aP < 0.05 vs. Q2.

^bP < 0.05 vs. Q2/Q3 (1:1).

^cP < 0.05 versus Q2/Q3 (0.5:1).