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. 2023 Jul 11;14:1191188. doi: 10.3389/fimmu.2023.1191188

Figure 1.

Figure 1

Microglia are the major sources for the upregulated Ccl2 after SCI. The quality control measurements taken to analyze altered genes associated with microglia following spinal cord injury (SCI). The data was obtained from the GEO database GSE45006. (A–E) GEO database GSE45006. (A) Principal component analysis (PCA). (B) Uniform Manifold Approximation and Projection (UMAP) plot. (C) Expression density analysis. (D) Volcano map displaying genes with significant differences, characterized by an adjusted p-value lower than 0.05 and a minimum 2-fold change. (E) List of highly altered genes after SCI with at least a 100-fold change. Arrow pointed to Ccl2. (F–I) Co-analysis with single-cell RNAseq data from SRA667466: SRS3059941. (F) tSNE layout of the cell clusters, noting that microglia were put in a red rectangle. (G–I) Co-analysis with Ccl2 (G), interleukin 33 (Il33, H) and regulating synaptic membrane exocytosis 1 (Rims1, I). Arrows pointed to major cell clusters expressing co-analyzed genes.