Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jul 11;14:1191188. doi: 10.3389/fimmu.2023.1191188

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Li, Yang, Wang, Chen and Shen

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Generation and validation of AAVs specifically targeting microglia and depleting Ccl2. (A) Development of plasmids expressing either a siRNA for Ccl2 (si-Ccl2) or a control scrambled sequence (Scr) under a microglia-specific TMEM119 promoter (pTMEM119-si-Ccl2; pTMEM119-si-Scr). The plasmids also contained a GFP reporter co-controlled by the TMEM119 promoter through a f2A linker. (B) Both plasmids were used to transfect a mouse microglia cell line (EOC2), a mouse macrophage cell line (J774a.1), and a mouse fibroblast cell line (3T3). The specificity of the TMEM119 promoter for microglia was confirmed as only the transfected EOC2 cells appeared to be green fluorescent in culture. (C, D) RT-qPCR for Ccl2 mRNA (C) and ELISA for Ccl2 protein (D) levels. *p<0.05. Scale bars were 100µm.