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Depletion of Ccl2 in microglia induces a proinflammatory to anti-inflammatory phenotypic adaption. (A) Assessment of growth of microglia transfected with either pTMEM119-si-Ccl2 or pTMEM119-Scr by a CCK-8 assay. (B, C) Cell invasion assay and migration assay by quantification (B) and by representative images (C, D) DCF assay. (E) Griess assay. (F) Arginase activity. *p<0.05. ns, non-significant. Scale bars were 100µm.
