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. 2023 Jul 11;14:1191188. doi: 10.3389/fimmu.2023.1191188

Figure 5.

Figure 5

Depletion of Ccl2 in microglia reduces chronic spinal compression-associated pain. The effects of microglial depletion of Ccl2 on chronic spinal compression and its associated pain were then examined in a mouse model (ttw) in vivo. Four groups of mice were included in this experiment and all were analyzed at 24 weeks of age. Group 1, wildtype mice of comparable age and gender (wildtype); Group 2: ttw mice (ttw); Group 3: ttw mice that had received orthotopic injection of AAVs carrying pTMEM119-Scr at 8 weeks of age; Group 4: ttw mice that had received orthotopic injection of AAVs carrying pTMEM119-si-Ccl2 at 8 weeks of age. (A) H&E staining at analysis. (B) The degree of the sickness was quantified by the ratio of spinal canal and cord are at site of maximal compression (mostly C2-C3) to those at the Th1 site. (C, D) The mechanical (C) and thermal (D) pain was evaluated by a Von Frey filament test. (E) The cold plate score in a cold plate test. (F) The paw licking duration in a Capsaicin test. *p<0.05. ns, non-significant. Scale bars were 100µm.