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. 2023 Jul 20;18:3989–4005. doi: 10.2147/IJN.S417445

Figure 4.

Figure 4

Cell viability and apoptosis of PANC-1 cells in vitro. (A) Schematic illustration of the Transwell co-culture system used to create a drug depot. (B) Cell viability of PANC-I cells incubated in the Transwell co-culture system was evaluated at 24, 48, and 72 hours. (C) Cell apoptosis of PANC-I cells incubated in the Transwell co-culture system was evaluated at 48 hours. (D) Cell apoptosis analysis measured by flow cytometry (stained by Annexin V-FITC/PI). (E) Representative fluorescent morphology images of the apoptotic PANC-1 cells were obtained after 48 hours of co-cultivation. Double-stained cells represent apoptotic cells. Scale bar is 200 μm. All data were obtained through three independent repeated experiments and presented as the mean ± SD. A significant difference was observed in the LSD post-hoc test. ***P < 0.001, **P < 0.01.