| Reagent | Final concentration | Amount (for 500 mL) |
|---|---|---|
| D-mannitol | 8.5% | 42.5 g |
| Calcium chloride (1 M CaCl2) | 10 mM | 5 mL |
| Distilled H2O | n/a | Add to 500 mL |
| Total | n/a | 500 mL |
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