| Reagent | Final concentration | Amount (for 20 mL) |
|---|---|---|
| D-mannitol 10.3% | 9.1% | 17.7 mL |
| Magnesium chloride (1M MgCl2) | 15 mM | 300 μL |
| MES | 10% | 2 mL |
| Total | n/a | 20 mL |
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