Reagent	Final concentration	Amount (for 250 mL)
Tris (1 M, pH 7.5)	200 mM	50 mL
NaCl (5M)	250 mM	12.5 mL
EDTA (0.5M, pH 8.0).	25 mM	12.5 mL
SDS	0.5%	0.0625 mL
Distilled H2O	n/a	Add up to 250 mL
Total	n/a	250 mL