Table 2.
Effects of the cryb mutation on BG-luc and tim-luc reporter activity in antennal specimens under light-dark cycles (LD 12:12), constant darkness (DD and constant temperature (HH)1,2
| Genotype3 | Na4 | Number5 Rhythmic (statistical significance) | Period (Mean ± SEM) | Rhythmicity6 Index (Mean ± SEM) | Rhythmicity Statistic (Mean ± SEM) | Rhythm Amplitude (Mean ± SEM) | Phase | Activity Counts (MeanfSEM) |
| LD | ||||||||
| BG-luc; cry+ | 36 | 33 (23) | 24.2 ± 0.3 | 0.49 ± 0.03 | 3.0 ± 0.2 | 0.17 ± 0.01 | 2.8, 0.9 | 335 ± 33 |
| BG-luc; cryb | 41 | 16 (3) | 25.8 ± 1.0 | 0.12 ± 0.06 | 0.8 ± 0.4 | 0.09 ± 0.02 | 8.2, 0.9 | 299 ± 65 |
| tim-luc; cry+ | 20 | 16 (9) | 25.0 ± 0.3 | 0.36 ± 0.05 | 2.3 ± 0.3 | 0.15 ± 0.02 | 2.1,0.9 | 309 ± 30 |
| tim-luc; cryb | 36 | 11 (1) | 26.5 ± 1.0 | 0.23 ± 0.05 | 1.4 ± 0.3 | 0.09 ± 0.01 | 9.2, 1.0 | 477 ± 105 |
| DD | ||||||||
| BG-luc; cry+ | 38 | 33(4) | 25.7 ± 0.4 | 0.20 ± 0.03 | 1.2 ± 0.2 | 0.12 ± 0.01 | 5.5, 1.0 | 404 ± 27 |
| BG-luc; cryb | 31 | 14(1) | 26.8 ± 1.3 | 0.03 ± 0.04 | 0.2 ± 0.2 | 0.02 ± 0.00 | 10.5,0.3 | 350 ± 75 |
| tim-luc; cry+ | 23 | 22(0) | 26.4 ± 0.4 | 0.12 ± 0.03 | 0.7 ± 0.2 | 0.08 ± 0.00 | 5.4, 0.8 | 269 ± 40 |
| tim-luc; cryb | 37 | 8(0) | 24.3 ± 2.2 | -0.1 ± 0.09 | -0.6 ± 0.5 | 0.04 ± 0.00 | 7.9, 0.5 | 625 ± 109 |
| HH | ||||||||
| BG-luc; cry+ | 112 | 94(5) | 25.4 ± 0.3 | 0.12 ± 0.01 | 0.7 ± 0.1 | 0.08 ± 0.00 | -0.1,0.6 | 487 ± 29 |
| BG-luc; cryb | 107 | 28(2) | 25.4 ± 0.5 | 0.14 ± 0.03 | 0.9 ± 0.2 | 0.06 ± 0.00 | 0.9, 0.6 | 624 ± 98 |
| tim-luc; cry+ | 112 | 74(0) | 25.3 ± 0.3 | 0.14 ± 0.01 | 0.8 ± 0.1 | 0.09 ± 0.00 | 0.2, 0.7 | 902 ± 73 |
| tim-luc; cryb | 80 | 37(4) | 25.8 ± 0.2 | 0.11 ± 0.02 | 0.7 ± 0.1 | 0.08 ± 0.00 | -1.4,0.5 | 674 ± 90 |
1) The experimental conditions are described in Krishnan et al [23] and in Materials and Methods. 2) Ambient conditions are light-dark cycles with 12 hours of light and 12 hours of darkness (LD 12:12) or constant darkness at 25°C or constant temperature in which the specimens were first synchronized to temperature cycles in constant darkness consisting of 12 hours of 18°C followed by 12 hours of 27°C before they were evaluated under a constant high temperature of 27°C (HH). See Materials and Methods for further details. Also see Krishnan et al. [23]3) All headings in Table 2 are defined as in Table 1. 4) The analysis was applied to samples previously described in Krishnan et al [23]. The number of specimens analyzed (Na) in LD and DD is unchanged, while the number of specimens in HH has increased by 123 additional cry+ antennal pairs and 62 additional cryb specimens (see Materials and Methods for more details) 5) We asked whether cryb affects rhythmicity in antennal pairs for each ambient condition. Chi-squared tests showed significant effects of the mutation on rhythmicity with either BG-luc or tim-luc in all cases (p < .001). 6) The cryb mutation significantly reduced the RI value for each reporter in LD and DD (t-test, p < .001) but not HH (p > .05).