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. 2023 Jul 3;11(3):30. doi: 10.3390/jdb11030030

Figure 5.

Figure 5

Topological distribution of putative stem cells in adult chicken reticulate scales and juvenile alligator tuberculate scales. (A) Strategy of TA cell labeling in adult chicken reticulate scales or juvenile alligator tuberculate scales. (B) Strategy of LRC labeling in adult chicken reticulate scales or juvenile alligator tuberculate scales. (C) H&E staining of adult chicken reticulate scales showing regions of interest. (H) H&E staining of juvenile alligator tuberculate scales showing regions of interest. The red and green rectangular boxes in panels (C,H) indicate the hinge region and surface region. (D–G) TA and LRCs in chicken reticulate scales. (D) 3 h pulse labeling. Red arrows indicate BrdU-positive cells. (E) BrdU 1-week labeling. (F) A 2-week chase period after 1 week of labeling. (G) An 8-week chase period after 1 week of labeling. (I–L) TA and LRCs in alligator tuberculate scales. (I) The 3 h BrdU pulse labeling. Red arrows indicate BrdU-positive cells. (J) BrdU labeling for 1 week. (K) An 8-week chase period after 1 week of labeling. (L) A 16-week chase period after 1 week of labeling. Blue arrows indicate the LRCs in the hinge region. Note that LRCs exist in the alligator tuberculate scale hinge regions but are negative in both the surface and hinge regions of chicken reticulate scales. d, dermis; e, epidermis; Hg, hinge; S, surface.