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Wound-healing assay and ImageJ calculation. Example of microscopic 10× image results of GF-containing medium scratch assays at time 0 h (A), 4 h (B), and 24 h (C). ImageJ scratch assay analyzer (MiToBo plugin) for time points 0 h (D), 4 h (E), and 24 h (F). Cell growth decreased the total area from 53.8% to 49.0% to 13.0% over the time period.)
