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. 2023 Jun 21;9(3):34. doi: 10.3390/ijns9030034

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Changes in AA concentrations upon long-term storage at room temperature. The boxplots represent the first quartile, median and third quartile. The whiskers extend to the 2.5th and 97.5th percentiles. Individual dots represent non-extreme outliers. Asterisks ‘*’ above boxplots represent statistically significant trends in the concentration upon storage duration, as determined by Jonckheere’s trend test. Samples from 2017 were stored at +4 °C (light grey box), while other samples transferred to storage at RT after one year (dark grey boxes). Trend analyses of Arg, His and Met could not be interpreted, because many measurements were below the detection limit. Assessment of Asp, Arg, Orn, Gln and Lys (indicated with grey background) was possibly less reliable due to high CVs.

Changes in AA concentrations upon long-term storage at room temperature. The boxplots represent the first quartile, median and third quartile. The whiskers extend to the 2.5th and 97.5th percentiles. Individual dots represent non-extreme outliers. Asterisks ‘*’ above boxplots represent statistically significant trends in the concentration upon storage duration, as determined by Jonckheere’s trend test. Samples from 2017 were stored at +4 °C (light grey box), while other samples transferred to storage at RT after one year (dark grey boxes). Trend analyses of Arg, His and Met could not be interpreted, because many measurements were below the detection limit. Assessment of Asp, Arg, Orn, Gln and Lys (indicated with grey background) was possibly less reliable due to high CVs.