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Timed anti-BMP treatments in ventralized embryos led to sequential fixation of anterior genes. The expression of xcg-1, six3, otx2, gbx2, and hoxd1 in bmp4-injected embryos that were subjected to Smad6 treatment at different stages. These experiments were repeated at least three times and >40 embryos were used for each condition. Black arrows indicate the anterior borders of the expression domains.
