Figure 2.

Epitope determination of C₄₄Mab-94 using deletion mutants of CD44v3–10. (A) The CD44v3–10 deletion mutants expressed on CHO-K1 cells. (B) The CD44v3–10 mutants-expressed CHO-K1 cells were incubated with C₄₄Mab-94 ((B), red line) or an anti-PA tag mAb, NZ-1 ((C), red line), or control blocking buffer (black line), followed by secondary antibodies treatment. The data were analyzed using the EC800 Cell Analyzer.