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Determination of C44Mab-94 epitope by ELISA. Four synthesized peptides, which cover the CD44v7 to v9 region, were immobilized on immunoplates. The plates were incubated with C44Mab-94 or buffer control (–1st Ab), followed by incubation with peroxidase-conjugated anti-mouse immunoglobulins. Optical density was measured at 655 nm. ELISA—enzyme-linked immunosorbent assay. NC—negative control (solvent; DMSO in PBS). Error bars represent means ± SDs.
