Figure 5.

Western blot analysis using C₄₄Mab-94. The cell lysates (10 µg) of CHO-K1, CHO/CD44s, CHO/CD44v3–10, HSC-3, MKN45, and NUGC-4 were electrophoresed and transferred onto polyvinylidene fluoride membranes. The membranes were incubated with 10 µg/mL of C₄₄Mab-94 (A), 10 µg/mL of C₄₄Mab-46 (B), and 0.5 µg/mL of an anti-β-actin mAb (C). Next, the membranes were incubated with anti-mouse immunoglobulins conjugated with peroxidase. The black arrows indicate CD44s (~75 kDa). The red arrows indicate CD44v3–10 (>180 kDa). CD44v8 was broadly detected in HSC-3, MKN45, and NUGC-4 lysates. The white arrow indicates a band at ~75 kDa recognized by C₄₄Mab-94 in the CHO/CD44v3–10 lysate. A colored marker was used in the analysis.