Fig. 2.

TAF15 maintained BRD4 mRNA stability in IL-1β-treated chondrocytes

(A) BRD4 mRNA level in cartilage tissues was detected by qRT-PCR (B) BRD4 expression level in chondrocytes following IL-1β treatment was assessed by qRT-PCR and Western blot (C–D) BRD4 expression in IL-1β-treated chondrocytes following si-NC or si-TAF15 transfection was determined by qRT-PCR and Western blot (E) RIP assay was carried out to analyze the interaction between TAF15 and BRD4 (F) BRD4 mRNA stability in chondrocytes after TAF15 knockdown was assessed using RNA degradation assay. Data were expressed as mean ± SD. All data were obtained from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.