Fig. 4.

BRD4 promoted BRD4 expression in IL-1β-treated chondrocytes (A–B) GREM1 mRNA level in cartilage tissues was detected by qRT-PCR and Western blot (C) GREM1 expression level in chondrocytes following IL-1β treatment was determined using qRT-PCR (D) BRD4 expression level in IL-1β-treated chondrocytes following si-NC or si-BRD4 transfection was determined using qRT-PCR and Western blot (E–F) GREM1 expression level in IL-1β-treated chondrocytes following BRD4 knockdown or BRD4 overexpression was determined using qRT-PCR and Western blot (G) RIP assay was carried out to analyze the interaction between BRD4 and GREM1. Data were expressed as mean ± SD. All data were obtained from three independent experiments. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.