Table 1. Sequences for homology-directed repair–mediated insertion of HiBiT-tag into C-terminus of Hexokinase isoforms.

Protein	sgRNA sequence	PAM	ssODN
HK2	ATAGAACCCCTGAAATCGGA	AGG	CTGCAGTCAGAGGATGGCAGCGGGAAGGGGGCGGCGCTCATCACTGCTGTGGCCTGCCGCATCCGTGAGGCTGGACAGCGAGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCTAGATCCCCTGAAATCGAAAGCGACTTCCTCTTTCTCTCCTTCTTCCCTGTTTTAAATTATAAGATGTCATCCCCTTGTGT
HK3	TCGTGTCTGAGGAAACCTCC	CGG	GATGGGTCCGGCAAAGGTGCGGCCCTGGTCACCGCTGTTGCCTGCCGCCTTGCGCAGTTGACTCGTGTCGTGAGCGGCTGGCGGCTGTTCAAGAAGATTAGCTGAGGAAACCTCCACGCTGAGGAGGTCTCCGCCGCAGCCTTGCTGGAGCCGGGTCGGGGTCTGCCTGTTTCCCAGCCAGGCCC