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. 2023 Jul 20;13(14):e4806. doi: 10.21769/BioProtoc.4806

Figure 3. Laser microdissection and recoil measurements.

Figure 3.

(A–B’) Tracheal terminal cell expressing the actin-binding domain of Utrophin fused to GFP (UtrABD-GFP) under btl-gal4. The black box is the ROI where the laser microdissection was or will be done. (A, A’, B) before laser cut; (A’’, B’) after laser cut. (A’) The blue box shows the region where a control particle image velocimetry (PIV) analysis was done, before the laser procedure (time points A vs. A’). (A’’) The red box shows the region where the PIV analysis was done comparing the images before and after laser ablation (time points A’ vs. A’’). Inset shows the graphical representation of the measurements exactly as generated by the plugin. (B’) After a second laser exposition in the same ROI, the sample generates an autofluorescence signal (arrowhead), evidence of phototoxicity. (C–C’’) Higher-resolution reconstructions of the insets shown in (A’–A’’). (C) Control PIV values (from images in A vs. A’). (C’) Experimental PIV values, before and after laser microdissection. (C’’) Color scale for vector values shown in (C–C’).