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. 2023 Jul 20;13(14):e4754. doi: 10.21769/BioProtoc.4754

Figure 2. Generation of giant unilamellar vesicles (GUVs) from lipid mixtures.

Figure 2.

(A, B) Preparation of the lipid mixture. The desired volume and type of lipid is mixed in chloroform:methanol (1:1; v:v) in a glass tube and (I) the solvent is evaporated in rotary evaporator under the reduced pressure of 250 mbar for 2–4 h; (II) the resulting thin lipid film on the glass tube is dissolved in chloroform:methanol (1:1; v:v) and (III) stored in a glass vial with screw caps sealed with parafilm. (B) The glass tube containing the lipids in chloroform is connected to the rotary evaporator. (C) Equipment for a GUV formation of the home-made chamber. (D) Schematic workflow of GUV formation by the swelling method. The lipid mixture is applied to the cleaned glass slides and (I) dehydrated in a desiccator at 250 mbar for 30–60 min. The dried lipid film is then (II) rehydrated in a swelling buffer for 2–4 h, resulting in the formation of giant vesicles.