Figure 4. Imaging the binding of fluorescent annexin V on giant unilamellar vesicles (GUVs).

Giant unilamellar vesicles are prepared from different lipids and incubated without (-) and with (+) annexin V in the presence of Ca²⁺. Vesicles are observed in bright light and annexin channel. DOPC, PC (18:1/18:1) only; DOPE, PC (18:1/18:1)/PE (18:1/18:1), (9/1, mol/mol); PS, PC (18:1/18:1)/PS (18:1/18:1), (9/1, mol/mol). Data shown are from one experiment representative of two independent vesicle preparations. Scale bar: 10 μm.