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. 2023 Jul 20;13(14):e4754. doi: 10.21769/BioProtoc.4754

Table 1. Confocal microscope settings.

Channel Laser Detector Scan speed Excitation, nm Intensity, % Emission, nm Gain

Annexin V

White light laser, intensity at 85%

Hybrid Detector

(HyD)

400 Hz 577 5 587–757 100

Bright light

White light laser, intensity at 85%

Photomultiplier

tube (PMT)

400 Hz trans channel - - 300