Figure 8. Example of the western blot analysis of opto-caspase-1-induced pyroptosis in U937 cells.

Experiment was performed as described above and treatment with nigericin, an NLRP3 inflammasome activator, was used as a positive control to detect IL-1β and GSDMD processing. Note the cleavage of opto-caspase-1 (as detected by the disappearance of full-length opto-caspase-1 band and accumulation of Cry2olig-mCherry), IL-1β, and GSDMD upon illumination. Figure adapted from Shkarina et al. (2022).