Figure 3. Infection procedure.

A. Material needed for infection procedure. B. Image of the size and packaging of the cotton pipe cleaner. C. The medical clay is added to the spores in an approximately 3:1 ratio. D. The clay is mixed with the spores by shaking the tube. E. The spore clay mix is poured on a black tile and picked up with the pipe cleaner. F. The barley plant is infected by carefully rubbing the pipe cleaner over the surface of the adaxial side of the leaf. G. The inner side of the pot covers is sprayed with Tween 20 solution. H. The moisturized cover cups are placed over the plants and fixed by pressing them slightly into the soil.