Figure 4. Spore harvesting procedure.

A. Prepare the 1 mL filter tips. Carefully heat and flatten the tip. Cut the flattened part of the tip to generate a flattened opening. B. Attach a tube with an inner diameter of approximately 8 mm to the vacuum pump and insert the tip at the end of the tube. C. Start the vacuum (between 500 and 700 mbar is normally sufficient). Carefully move the spore hoover over the leaf surface to collect the spores in the filter tip. D. For drying, store the collected spores in the tips in an exsiccator filled with silica beads. E. Tightly close the lid of the exsiccator with parafilm during storage at 4 °C. F. 1 mL filter tip with harvested spores and silica beads, closed with parafilm.