| Reagent | Final concentration | Amount |
|---|---|---|
| NaCl | 1.4 M | 40.91g |
| Na2HPO4·2H2O | 10 mM | 0.89 g |
| KCl | 27 mM | 1.013 g |
| KH2PO4 | 18 mM | 1.23 g |
| Dissolved in ddH2O | n/a | 500 mL |
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