Figure 8. Stimulation protocol for paired-pulse stimulation of the cell patched with probe 1 and postsynaptic response recording from the cell patched with probe 2.

Stimulation protocol represents a series of stimulations by two sequential depolarizations from -70 mV to 60 mV (5 ms duration each) with increasing inter-pulse interval. To stimulate cells on probe 2 with the response recording on probe 1, replace values in “DA” and “AD” sections (1 into 2, 2 into 1).