| Reagent | Final concentration | Amount |
|---|---|---|
| K-gluconate | 125 mM | 585.62 mg |
| KCl | 10 mM | 14.9 mg |
| HEPES | 10 mM | 47.66 mg |
| MgCl2·6H2O | 1 mM | 4.06 mg |
| EGTA solution, 0.5M | 2 mM | 80 μL |
| ATP (Mg) | 4 mM | 40.57 mg |
| GTP (Na) | 0.3 mM | 3.14 mg |
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