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. 2023 Jul 20;13(14):e4723. doi: 10.21769/BioProtoc.4723

Figure 12. Inspection of the subtomogram average of the full-length microtubule.

Figure 12.

(A) MV Controls menu. (B) Selection of the 81 particle subtomogram average (MT_AvgVol_81.mrc) in the ZaP window. (C) Visualization of the 81 particle subtomogram average showing a B-type lattice organization. (D) Rotation of the map by 180° around the Y-axis. (E) Visualization of the 81 particle subtomogram average showing two seams of the A-lattice type and the middle protofilament with thinner densities with respect to the adjacent protofilaments.