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. 2023 Jul 20;13(14):e4724. doi: 10.21769/BioProtoc.4724

Figure 1. Genomic landscape of potential targets in exon 2 and 3 of wildtype PD-L1 with possible guide sequences.

Figure 1.

Sequence of exon 2 and 3 from CD274 with corresponding open reading frames (ORF) (+1) as depicted in CLC Genomics Workbench (dark blue). Exons are depicted in blue and start codons for ORF+1 are depicted in yellow and purple (ATG1 and ATG2, respectively). Possible guide sequences for targeting crRNA are depicted in lilac. Alternative stop codon (TGA ORF+2), 72 bp downstream of guide_61/fw_ex3-induced cutting site (in between A and C, on position 57 and 58 of exon 3), is depicted in red.