Skip to main content
. 2023 Jul 20;13(14):e4724. doi: 10.21769/BioProtoc.4724

Figure 5. Confirmation of +1 frameshift in founder pups via Sanger Sequencing.

Figure 5.

The alignment of the sequencing data to the amplicon shows a clean incorporation of the insertion template with the HindIII restriction site AGCT into the genome of founder pup number 10 (10_premix.scf).