Figure 6. Routine genotyping by PCR and HindIII digestion.
C- shows negative control (PCR and HindIII restriction reaction without DNA template); -/- shows homozygous mutant with 200 bp fragments; +/- shows heterozygous mutant with both 200 bp and 400 bp fragments; +/+ shows wildtype gene with 400 bp fragments; L is the DNA ladder.