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. 2023 Jul 20;13(14):e4724. doi: 10.21769/BioProtoc.4724

Figure 6. Routine genotyping by PCR and HindIII digestion.

Figure 6.

C- shows negative control (PCR and HindIII restriction reaction without DNA template); -/- shows homozygous mutant with 200 bp fragments; +/- shows heterozygous mutant with both 200 bp and 400 bp fragments; +/+ shows wildtype gene with 400 bp fragments; L is the DNA ladder.