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Mouse peripheral blood mononuclear cells (PBMCs) were stimulated overnight with 10 ng/mL IFNγ and consequently surface-stained for PD-L1. (A) Gating strategy. (B) Representative overlay of histograms of PD-L1 expression of CD45+ immune cells as gated in A), showing PD-L1-/- cells in black (-/-) and wildtype cells in red (+/+).
