Table 1. Oligos and primers.
| Name | Application | Sequence (5′–3′) | Length (bp) | Manufacturer |
|---|---|---|---|---|
| guide_61/fw_ex3 | crRNA | GTATGGCAGCAACGTCACGA | 20 | Alt-R CRISPR, iDT |
| tracrRNA | tracrRNA | Sequence according to manufacturer | 67 | Alt-R CRISPR, iDT |
| HDR template HindIII +1fs | ssDNA homology-directed repair donor template | CGTTTACTATCACGGCTCCAAAGGACTTGTACGTGGTGGAGTATGGCAGCAACGTCAAGCTTGGAGTGCAGATTCCCTGTAGAACGGGAGCTGGACCTGCTTGCGTTAGTG | 111 | Megamer, iDT |
| guideRNA_61/fw_Left | Fw Primer | CCCCGCCCCATGAAGTTATT | 20 | Microsynth |
| guideRNA_61/fw_Right | Rv Primer | TGCAGCTTGACGTCTGTGAT | 20 | Microsynth |