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| Reagent | Concentration | Volume (25 μL total/reaction) |
|---|---|---|
| guideRna168forwLe | 10 μM | 1.25 |
| guideRNA168forwRi | 10 μM | 1.25 |
| dNTPs | 200 μM | 0.5 |
| 10x buffer | 2.5 | |
| Taq polymerase | 2.5 U | 0.125 |
| ddH2O | 18.375 | |
| DNA | 1 |
