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. 2023 Jul 20;13(14):e4724. doi: 10.21769/BioProtoc.4724

Table 3. PCR Conditions.

Step Time (s) Temperature (°C)
1 60 98
2 10 98
3 30 65
4 30 72
Go to step 2 35×
5 120 72
6 Up until use 4