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. 2023 Jul 20;13(14):e4722. doi: 10.21769/BioProtoc.4722

Table 1. List of primary and secondary antibodies.

Target Species Dilution Catalogue number Supplier
Anti-E-cadherin Rabbit 1:500 24E10 Cell Signaling Technology
Anti-Sox2 Rabbit 1:400 ab97959 Abcam
Anti-Sox1 Goat 1:50 af3369 R&D Systems
Anti-Otx2 Goat 1:25 af1979 R&D Systems
Anti-Brachyury Goat 1:300 sc-17745 (C-19) Santa Cruz
Anti-Brachyury Rabbit 1:100 ab209665 Abcam
Anti-Oct4 Mouse 1:200 sc-5270 (C-10) Santa Cruz
Anti-Nanog Rat 1:300 14-5761-80 Thermo Fisher
Anti-Cdx2 Rabbit 1:200 ab76541 Abcam
Anti- aPKC Mouse 1:100 sc-17781 (H-1) Santa Cruz
Anti-Sox17 Goat 1:200 AF1924 Abcam
Anti-Cdx2 Rabbit 1:200 ab76541 Abcam
Anti-mCherry Rat 1:400 M11217 Thermo Fisher
Phalloidin AF488 1:1,000 A12379 Thermo Fisher
Phalloidin AF635 1:1,000 A34054 Thermo Fisher
Anti-chicken Alexa Fluor 488 Donkey 1:500 703-545-155 Jackson ImmunoResearch
Anti-rat Alexa Fluor 568 Goat 1:500 A-21247 Thermo Fisher
Anti-goat Alexa Fluor 568 Donkey 1:500 A-11057 Thermo Fisher
Anti-goat Alexa Fluor 647 Donkey 1:500 A-21447 Thermo Fisher
Anti-rabbit Alexa Fluor 488 Donkey 1:500 A-21206 Thermo Fisher
Anti-rabbit Alexa Fluor 568 Donkey 1:500 A-10042 Thermo Fisher
Anti-rabbit Alexa Fluor 647 Donkey 1:500 A-31573 Thermo Fisher
Anti-mouse Alexa Fluor 568 Donkey 1:50 A-10037 Thermo Fisher
Anti-mouse Alexa Fluor 647 Donkey 1:50 A-31571 Thermo Fisher