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. 2023 Jul 11;10:1204740. doi: 10.3389/fmolb.2023.1204740

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Copyright © 2023 Chen, Tang, Hou, Luo, Chen, Qiu, Chen, Li, He and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Verification of the expression of significantly dysregulated tsRNAs by real time-PCR. We selected 8 tsRNAs dysregulated in PAH for custom real time-PCR. (A) Expression of 4 upregulated tsRNAs in PAH. (B) Expression of 4 downregulated tsRNAs in PAH. ***p < 0.0001 as indicated. (C) Examples of tRNA or pre-tRNA structures of the eight validated tsRNAs were depicted. The red nucleotides indicate the presence of the eight validated tsRNAs sequences. Exp, PAH patients; Con, healthy controls.