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A) After opening the chosen picosirius red–stained image, set the image type to Brightfield Other. B) Example of three annotations used for the Fibrosis intensity script to gather an average intensity of picosirius red staining. C) The Fibrosis detection script will automate tissue annotation and D) differentiate between positive (fibrotic septa-red) and negative (parenchymal-blue) staining. B and C, scale bars: 2 mm. D, scale bars: 50 μm.
