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. 2023 May 18;18(7):1523–1533. doi: 10.1021/acschembio.3c00141

Figure 5.

Figure 5

Comparison of intensity and FLIM phasor processed images of HeLa cells stained with 1c (20 μM). Separation of lifetimes was performed using FLIM phasor analysis, in which very short lifetimes appear on the bottom right and very long lifetimes appear on the bottom left. The phasor plot shows four unique lifetimes (color-coded on the FLIM image), corresponding to fluorophores within the nucleolus, nucleus, cytoplasm, and surrounding media. Scale bar: 10 μm.