. 2023 May 18;18(7):1523–1533. doi: 10.1021/acschembio.3c00141

Table 1. Photophysical Properties of Styrene-Based Fluorescent Molecules.

probe	solvent^a	λ_abs^b	ε^c	λ_em^d	Stokes shift (nm)	Φ_f^e^,^f(%)	fluorescence enhancement
1a	DMSO	436	29400	592	156	2.1
	T.E.	422	20600	584	162	0.12	1
	RNA	434	19300	586	152	9.9	83
1b	DMSO	426	35400	570	160	1.3
	T.E.	410	24400	568	142	0.12	1
	RNA	416	23300	552	136	3.9	33
1c	DMSO	518	36000	606	88	2.0
	T.E.	510	27500	596	86	0.10	1
	RNA	556	27500	608	76	49	490
1d	DMSO	496	40300	586	90	3.3
	T.E.	486	34600	572	86	0.38	1
	RNA	502	29400	584	82	50	132
MPI	DMSO	440	30500	538	98	3.7
	T.E.	430	26500	540	110	0.52	1
	RNA	442	21200	534	92	32	62

DMSO: dimethyl sulfoxide. T.E. buffer: 10 mM Tris–HCl, 1 mM EDTA, pH = 7.5. RNA: 200 μg/mL type IV torula yeast.

Absorbance maximum wavelength (nm).

Molar absorptivity (M^–1 cm^–1).

Fluorescence maximum wavelength (nm).

Fluorescence quantum yield of dyes using Coumarin 6 (Φ500 =0.78) and Rhodamine 6G (Φ548 = 0.95).

Fluorescence quantum yield of dyes incubating with nucleotides using integrating sphere. Quantum yield data were averaged from duplicate experiments.