Figure 2.

rAaeUPO-catalyzed oxidation of cyclohexane on a 10.9 L scale. Two individual batches are shown (black, red). (black circle, red circle): total product formed; (black triangle, red triangle): H₂O₂ concentration. Reaction conditions: 500 mM substrate (starting) concentration, 50 vol % ACN, 100 mM potassium phosphate buffer, pH 6.0, 20 μM AaeUPO (concentrated supernatant), H₂O₂ dosing rate: 120 mL h^–1 from 12.75 M stock, (pump flow rate was 2 mL per min). The reaction was stirred at 225 rpm at 25 °C. The product concentration was calculated from calibration curves. GC analysis on achiral column (CP-Wax 52GB).